The demonstrators assembled at the Garden of Remembrance at 1pm before marching down Dublin’s O’Connell Street to the Irish Water offices on Talbot Street.
Campaign groups from around the country descended on the capital for the march.
The protests come as the company issued its first bills this week.
“On this symbolic day that our bills will be arriving we will march to Irish Water head office,” said organiser Michael McDermott from the Cavan Says No To Water Charges group.
“We had to mark the day with some presence. We couldn’t let it go unnoticed," he added.
He also revealed plans to stage more protests in the
“Every day that Irish Water give out bills in the next few months we will launch another march to the headquarters," he added.
Following the march, a number of protesters gained entry to the Irish Water head office where they occupied it for a time.
In a statement, the group said: “We took non-violent direct action because we believe marching, speeches and elections alone will not create the required change.
“Civil disobedience has been at the forefront of the Irish Water resistance and has proven throughout history to be an effectivemethod of creating change.”
Also on Wednesday, the Anti-Austerity Alliance’s Non-Payment Network launched their plans for a national ‘Bin Your Bills’ protest on Saturday 18 April.
Speaking outside the Irish Water head office, Paul Murphy of the Anti-Austerity Alliance told protesters that “mass non-payment can defeat Irish Water and the government”.
The group say they are hoping to build non-payment of bills “as the key to forcing the abolition of the water charges”.
The planned march, from the Garden of Remembrance at 2pm on 18 April, follows a national demonstration against water charges in Dublin less than a fortnight ago.